stat3  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Antibody Transcytosis and Neutralizing Activity in Respiratory Epithelial Cells

doi: 10.64898/2026.05.25.727697

Figure Lengend Snippet: A) Immunoblot of FcRn and pIgR in hNECs and hBECs. B, C) Relative protein expression levels by Western blotting of FcRN, pIgR, normalized to β-tublin, n = <3. D, E) Quantification of flow cytometry cell types by percentage of total cells, values are expressed as mean ± SE, n = 1/group. F, G) Transcytosis of IgG and IgA was determined using an ELISA assay. Values are expressed as mean ± SE, n = <3well/time point. *P<0.05 **P<0.01

Article Snippet: Human nasal epithelial cells (hNECs) or bronchial epithelial cells (hBECs) (Promocell) were grown to confluence in 24-well Falcon filter inserts (0.4-uM pore; 0.33cm 2 ; Becton Dickinson) using PneumaCultTM-Ex Plus Medium (Stemcell, Cat# 05001).

Techniques: Western Blot, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: The Joubert syndrome protein CSPP1 is a conserved regulator of vertebrate multiciliogenesis and motile cilia function

doi: 10.64898/2026.03.20.713242

Figure Lengend Snippet: (A) Schematic illustration of multiciliated cell (MCC) differentiation in mouse tracheal epithelial cells (MTECs) and Xenopus embryonic epidermis. Key stages include cell cycle exit, centriole amplification (fibrous granules and deuterosomes), basal body docking, and ciliary axoneme elongation. ( B–F) CSPP1 localization in Mouse Tracheal Epithelial Cells (MTECs). Representative immunofluorescence images of MTECs at different air-liquid interface (ALI) culture days. Scale bars: 5 μm. (B) Centrioles. Cells were stained for CSPP1 (green), Centrin (magenta; centrioles), and ZO-1 (yellow; tight junctions) across differentiation stages. (C) Fibrous granules. MTECs at ALI2 (amplification) stained for CSPP1 (green), PCM1 (magenta; fibrous granules), and Centrin (yellow). (D) Deuterosomes. At ALI2, CSPP1 (green) associates with deuterosome cores marked by DEUP1 (magenta), surrounded by Centrin-positive nascent centrioles (yellow). Inset in ALI2 is 2.5X zoom. (E) Proximal-distal orientation at the centrioles. Top (ALI2) and Bottom (ALI8): CSPP1 (green) localizes relative to ODF2 (magenta; mother centriole appendage) and gamma-tubulin (yellow). Cyan arrowheads indicate the mother centriole (MC); DC indicates daughter centriole. Inset in ciliary plane is 2.5X zoom. (F) Basal bodies and cilia (ALI8). Orthogonal views show CSPP1 (green) localizing to the basal body plane (co-stained with Centrin, yellow) and extending into the ciliary plane (co-stained with Acetylated Tubulin, magenta). Inset in ciliary plane is 3X zoom. (G–J) Cspp1–mCherry localization in Xenopus epidermal MCCs. Representative immunofluorescence images of Xenopus MCCs at different differentiation stages. Scale bars: 10 μm (main panels), 0.1 μm (insets). (G) Fibrous granules. During early amplification, Cspp1–mCherry (magenta) co-localizes with PCM1 (green) and Centrin (yellow) within fibrous granules. Intercalating cell is outlined by dashed lines. Insets are 10X zoom. (H) Deuterosomes. During active amplification, Cspp1–mCherry (magenta) associates with deuterosomes marked by Deup1 (top, green) and PCNT (bottom, green), surrounded by Centrin-positive nascent centrioles (yellow). Intercalating cells are outlined by dashed lines. Inset is 10X zoom. (I) Basal bodies. In differentiated MCCs, Cspp1–mCherry (magenta) localizes to basal bodies marked by PCNT (top, green) and gamma-tubulin (bottom, green), occupying a region partially distinct from the Centrin core (yellow). Inset is 10X zoom. (J) Cilia. Cspp1–mCherry (magenta) localizes to the ciliary axoneme marked by Acetylated Tubulin (green) with enrichment at the distal tips, while maintaining basal body localization (bottom panels; Centrin in yellow).

Article Snippet: Cryopreserved human bronchial epithelial cells (HBEpC, C-12640, PromoCell, maximum passage 3) were thawed in T75 flasks in complete PneumaCult-Ex Plus medium and cultured at 37°C with 5% CO2.

Techniques: Amplification, Immunofluorescence, Staining